- Albany Medical Center (Albany, NY)
- …General Work Shift: Day (United States of America) Salary Range: $0.00 - $0.00 GRNA The intent of this position is to allow those students who have graduated ... Center Nurse Anesthesiology Program to be employed within 30 days of graduation as a GRNA prior to certification as a CRNA. The employee shall take and pass the… more
- Eurofins US Network (Boston, MA)
- …media preparation. + Develop and optimize CRISPR-based gene-editing workflows, including gRNA design and delivery. + Generate and characterize clonal gene-edited ... iPSC lines using flow cytometry, PCR, ddPCR, dPCR, and NGS. + Execute directed differentiation of iPSCs into pancreatic islets and assess drug substance parameters (cell counts, viability, metabolite measurements). + Support assay development for… more
- Eurofins US Network (Boston, MA)
- …to enable GMP readiness. + Develop and optimize gene-editing workflows, including gRNA design, RNP delivery, knock-in cargo design, clone genetic screening, and cell ... banking. + Characterize edited iPSC clones using DNA-based assays (PCR, qPCR, ddPCR), sequencing, flow cytometry, and next-generation sequencing (NGS). + Evaluate and implement iPSC culture media optimization and gene editing strategies to ensure genomic… more
- Eurofins US Network (Boston, MA)
- …and media making. + Optimize workflows for iPSC CRISPR based gene-editing, gRNA screening, and delivery. + Optimize and perform assays for on-/off-target analysis ... via PCR and NGS workflows. + Generate clonal gene edited iPSC lines and characterize via flow cytometry, ddPCR, dPCR and qPCR. + Culture and execute routine directed differentiation of human iPSCs to pancreatic islets performing drug substance assessments such… more
- Lilly (Boston, MA)
- …and phase-appropriate qualification and validation of analytical assays for the analysis of gRNA , mRNA, and LNP. Work in partnership with internal and/or external QC ... to coordinate analytical method transfer within the organization or at Contract Manufacturing Organizations (CMOs)/Contract Testing Laboratories (CTLs). + Author, review, and revise technical source documents to support phase-appropriate comparability. + Serve… more
- Lilly (Boston, MA)
- …+ Drive technical readiness and on-site support for manufactures of mRNA and gRNA across early-stage and late-stage programs. This includes batch record writing and ... executed record review, process scale-up, process data trending and support of quality activities including change controls, deviation investigations, implementation of process improvements. Preference for candidates familiar with early- and late-stage… more
- Lilly (Boston, MA)
- …literature and state-of-art practices and applying these learnings to Verve's gRNA process development projects. + Collaborate cross-functionally with process and ... analytical teams to progress process and manufacturing technology development projects + Review and provide technical feedback on internal and external collaborators experimental designs and results + Participate actively in technical discussions and meetings,… more
- Gilead Sciences, Inc. (Foster City, CA)
- …acid payloads. This includes mRNA, DNA and components for gene editing (eg, gRNA , donor DNA) to optimize payload function and stability. + Develop and execute ... a suite of robust in vitro cell-based assays to quantify payload delivery (transfection efficiency), protein expression and functional outcomes (eg, gene editing efficiency, target knockdown) in relevant cell lines and primary cells. + Characterize the… more